Home > Sandwich ELISA Kits > Human Granulocyte Chemotactic Protein 2 (GCP2) ELISA Kit

Product Name:

Human Granulocyte Chemotactic Protein 2 (GCP2) ELISA Kit

Cat.#:

JL12556

Regulatory Status:

RUO

Alternative:

CXCL6; SCYB6; GCP2; CKA-3; Chemokine(C-X-C-Motif)ligand 6; Small Inducible Cytokine Subfamily B(Cys-X-Cys)Member 6; Chemokine alpha 3; Small-inducible cytokine B6

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

15.62-1000pg/mL

Sensitivity:

7.65pg/mL

Specificity:

This kit recognizes Human GCP2 in samples. No significant cross-reactivity or interference between Human GCP2 and analogues was observed.

Sample Type:

serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Procedure:

Research Area:

Immunology

Size & Price:

Human A1BG Polyclonal Antibody

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Product Datasheets

Instruction:

Data
Citations
Sequence / 3D

| GCP2

Chemokine (C-X-C motif) ligand 6 (CXCL6) is a small cytokine belonging to the CXC chemokine family that is also known as granulocyte chemotactic protein 2 (GCP-2). As its former name suggests, CXCL6 is a chemoattractant for neutrophilic granulocytes.It elicits its chemotactic effects by interacting with the chemokine receptors CXCR1 and CXCR2.

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

Concentration (pg/mL)	1000	500	250	125	62.5	31.25	15.62	0
OD	2.31	1.65	1.06	0.69	0.44	0.32	0.23	0.1
Corrected OD	2.21	1.55	0.96	0.59	0.34	0.22	0.13	-

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

| Recovery

The recovery of Human GCP2 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	84-101	96
Plasma(n=8)	92-105	102
Cell Culture Supernates(n=8)	96-108	105

| Linearity

Samples were spiked with high concentrations of Human GCP2 and diluted with Universal Diluent to produce samples with values within the range of the assay.

		Serum(n=4)	Plasma(n=4)	Cell Culture Supernates(n=4)
1 : 2	Range (%)	84-95	88-96	90-110
1 : 2	Average (%)	91	93	96
1 : 4	Range (%)	89-103	87-108	105-115
1 : 4	Average (%)	94	98	108